Ryan_Shon

 Nycodenz (Axis-shield) 500g  ₩1,000,000 Copyright: Accurate chemical , edited by RS_

 DNase I (Roche)      can use DNase I (biosesang, D1027) Copyright: Roche , edited by RS_

 Pronase (Roche) can use Protease (Sigma-Aldrich, cat. no. P5147) Copyright: Roche , edited by RS_

Collagenase Roche Related with  Primary hepatocyte isolation Copyright: Roche  edited by RS_

  Worthington   Collagenase Product Information  Related with     Primary hepatocyte isolation Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca 2+  is required for enzyme activity. Particular enzymatic profiles o...